Extraction of GAPDH Gene from Grass Species

origination of GAPDH Gene from Grass SpeciesThe extraction of the GAPDH gene from grass species, for sequencing, to determine which grasses volition be most suitable for the stimulation of hydrocarbon-degradationIntroductionGlyceraldehyde-3-phosphate gehydrogenase gene (GAPDH) is a house keeping gene for a crucial enzyme which catalyses an important meter in glycolysis, found in all phylogeny. This gene can be extracted and isolated from plant gDNA through the use of PCR. Once cloned, the GAPDH gene get out be sequenced and ultimately analysed through bioinformatics for further research. The grasses that will be investigated throughout this experiment are as follows.Cymbopogon Ambiguous, also comm just known as lemon scented grass, mainly grows in NSW, QLD, NT and WA. This grass is a topknotted perennial, which has thin dark green coloured leaves, growth 60-100cm tall approximately.As the common name for this grass states, when this grass is crushed it gives off a straightforw ard lemon smell, allowing it to be easily identified. This grass is also kinda a hardy grass, it is rattling tolerant to wind, drought and frost conditions, allowing it to be grown in a variety of different areas.Themeda Triandra, known as Kangaroo Grass is native to Australia, found to be growing in all state and territory (Unkown, n.d. Native Seeds). This grass is a tufted perennial, growing up to 1.5 m tall and 0.5 m wide. This particular grass is sooner coarse, with a grey/green leaves, which produce a very distinct red/brown spikelets (Jennifer Liles, 2004) with the head containing the seeds.Themeda Triandra is considered a good grass for daily grazing however it does not tolerate heavy or incessant grazing. Meaning it can be a good indicator for the level of animal activity in a certain grassland area, as if browse too frequently, the grass has been known to disappear, and not re-grow.Dicantheum Carrisium, known as Queensland sick Grass is a native Australian Grass, gro wn not only in Queensland, but all parts of Australia. This Grass was originally very popular in Southern Queensland, due to its optimum performance in the weather conditions of the area.Dicantheum Carrisium is a perennial, with an upright tufted appearance. Growing from 30-80cm tall, and 10-15cm wide with compressed broad leaves, It is quite a soft texture with typically puritanic/dark green colour, making it quite distinguishable from numerous other grasses.Microlaena Stipoides, known as Weeping Grass, is native to Australia and is found in many of the damper regions from Cape York Town to Tasmania (Unknown, n.d. Native Seeds).Microlaena Stopoides is a slowly dispersal perennial, growing to approximately 70cm tall, with weeping seed heads. This is a very punishing and tolerant grass, suited to many different pH soil levels